We pursue professional technology for all infertility couple.
Equipped with the latest and highly practical equipment, including the next generation sequencing, NGS, biochip microarray laser scanner, and PCR system, our laboratory offers secure and precise testing services of reproductive medicine based on technical expertise and high-specification equipment. The major detections cover preimplantation genetic diagnosis and preimplantation genetic screening, boosting the probability of implantation and conception.
● Preimplantation Genetic Screening (PGS)
⋙ Couples with the following conditions are advised to undertake PGS for increasing conception rate：
- Women of advanced maternal age (females over 35 years of age)
- Habitual abortion
- Multiple failures of embryo implantation
- Chromosomal abnormalities or chromosomal translocation in family history
- Anyone who wishes to eliminate chromosomal disorder
- Patients with inherited disease (for example chromosomal translocation)
PGS Executed by Next Generation Sequencing, NGS
● Preimplantation Genetic Screening, PGS
Studies show that the abnormal structure or number of chromosomes carried by an embryo may result in habitual abortions and infertility of advanced maternal age or other unknown causes. Through the latest technology of preimplantation genetic screening, PGS, embryos with normal chromosome number can be selected for implantation. It can abate spontaneous or artificial abortions derived from chromosome abnormalities and increase the success rate of in vitro fertilization. Other than adopting Array-CGH, we have imported the cutting-edge technique of next generation sequencing, NGS for our PGS to offer fast and precise genetic analysis with high resolution.
● Procedure of Preimplantation Genetic Screening (PGS)
- Visit outpatient service for genetic counseling and proceed to in vitro fertilization procedure.
- Conduct embryo biopsy and embryo refrigeration on the fifth/sixth day after egg harvesting.
- Carry out preimplantation genetic screening.
- Select embryo of normal genes and implant during the next cycle.
● Preimplantation Genetic Diagnostics, PGD
For patients with familial hereditary diseases, a cell from eight-cell embryos is obtained for analysis through PGD before implantation in order to avoid abnormal genetic genes being inherited by the next. Our hospital offers customized services where we design personal testing strategies and methods conforming to each patient’s condition.
● Preimplantation Genetic Diagnosis（PGD）
⋙ Targeting single gene disorder or chromosome translocation, the preimplantation genetic diagnosis helps to screen healthy embryos to boost the probability of implantation and conception.
- Genetic test for alpha or beta polypeptide thalassemia
- Genetic test for Third type spinocerebellar atrophy
- Genetic test for polycystic kidney disease
- Fabry disease
- Ankylosing spondylitis (AS)
- Customized testing services for household heredity factors
- Abnormal chromosome number or irregular structure of chromosomal translocation
● In vitro Fertilization, IVF
In vitro fertilization, IVF is commonly known as test-tube babies. The ovum and sperm will placed in a petri dish for the purpose of achieving fertilization in the embryo laboratory.
● Intra-cytoplasmic Sperm Injection, ICSI
Targeting patients incapable of normal fertilization, the use of intra-cytoplasmic sperm injection, ICSI applies micromanipulation to infuse vital spermatozoa of normal appearance into the mature egg for the purpose of achieving fertilization.
● IVF Implantation
The normally developed embryo cultured in vitro will be selected and implanted into the uterus through the vagina and cervix at the appropriate timing according to the clinical status and demand of the patient after insemination. It is especially applicable to women with the problem of bilateral oviduct obstruction and adhesion, or infertile women who fail to become pregnant after undergoing repetitive intrauterine inseminations.
● The Dynamic Analysis of Embryo Development (Embryogenesis)
We utilize a real-time image surveillance system of embryo development (called Embryoscope Time-lapse System) to take photographs once every ten minutes during the fertilizing period, monitoring the developmental condition of the embryo continuously and observing its growth by means of dynamics analysis software before implantation. This technique not only distinguishes the embryonic appearance but also provides abundant embryo division dynamic information, which is used to select the best embryo for implantation while enhancing the probability of gestation.
- Increase success rate of implantation while lowering the possibility of abortion.
- Provide more information of the embryo and raise fine-scale selection rate
- Offer the embryo a more stable growing environment for better developmental conditions.
- The standardization of evaluation possesses multiple data references which are more reliable and trustworthy.
● Laser Assisted Hatching, LAH
The use of the assisted hatching (AHA) technique to segment a small hole in the zona pellucida helps the embryo implanted in women after implantation in the endometrium. To females of advanced maternal age or those who have experienced repetitive failures of in vitro fertilization, the study has confirmed that this technique is capable of increasing the chance of conception. The hospital uses a laser assisted hatching method, which can quickly, accurately and safely perforate the egg zona pellucida.
Proper Execution of Laser Cutting of Zona Pellucida
Enlarged Drawing of Laser Assisted Hatching
● Ovum Spindle Identification Technology
The spindle plays an essential role in the process of cell division. It traverses the movement of chromosome and will lead to chromosome abnormality or directly affect the embryonic development if the spindle is damaged. For dealing with the phenomena of spindle deviation in women of advanced maternal age, we utilize polarized light microscopy to identify the chromosomal location, to avoid damage of sperm chromosome during microinjection and achieve the goal of fertilization smoothly.
Proceeding Spindle Identification and Analysis in a Non-invasive Way
● Defrosting Technique for Frozen Embryo
We use the newest-generation vitrification cryopreservation and defrosting equipment to increase the survival rate of an embryo’s long-term preservation in liquid nitrogen.
Detection of Gene Mutation Point
● Sperm analysis
Regular sperm analysis is conducted to decide whether or not the sperm is in normal condition. The test items involve the volume (2 to 5 ml in normal), quantity and concentration (normally > 15 million/ml) of the sperm, sperm avidity (normally > 40%), sperm motility (normally > 32%), sperm viability/survival rate (normally > 58%), the proportion of regular sperm appearance (normally > 4%), the amount of leukocyte/white blood cell containing in spermatozoa (normally < 1 million/ml), and the possible agglutination phenomenon of sperm, and so on.
● Computer-assisted Sperm Analysis, CASA)
CASA is a set of full-automated inspection equipment designed for sperm examination. It is an analysis system combining computer technology with image recording that is capable of providing accurate, precise, prompt, and complete analysis reports of spermatozoa on its quantity, avidity, motility, linearity, and oscillation amplitude, etc. Currently, apart from the function of determining the activity of sperm, the analysis software for sperm motility has already been updated to an automatic identification design targeting sperm morphology.
● Hyaluronan Binding Assay, HBA)
It is a functional test exclusively for spermatozoa adopted to predict the fertilizing ability of sperm. The human ovum released from the follicle is wrapped by cumulus cells whose cumulus extracellular matrix is principally composed of an element called Hyaluronan, HA. During the process of natural insemination, the Hyaluronan, a chemical factor that attracts the integration of sperms, will induce the hyperactivation of spermatozoa where only the mature ones are allowed to compound with Hyaluronan. Accordingly, we can discriminate sperm maturation and normal physiological function based on the binding rate of sperm and Hyaluronan (normally > 18%) calculated by HBA. It can be exploited for further anticipation of the sperm’s fertilization ability toward the ovum in clinical practice.
Analysis Set of Sperm Hyaluronan Combination
Binding Efficiency Analysis of Sperm and Hyaluronan Integration
● Sperm Purification, Refrigeration, and Defrosting
Targeting subjects such as husbands who participate in intrauterine insemination, sperm donors, male patients with hereditary disease, preventative sperm storage for any contingency (for example unexpected or accidental events), sperm preservation for cancer patients before their undertaking chemotherapy or radiation therapy, or sperm conservation before ligation, the active sperm from semen will be segregated and purified through the pure sperm wash procedure. Sperm in need of long-term preservation will be stored in liquid nitrogen after being processed by an appropriate cooling method and cryo-protectant (cryo-protective agent). When necessary, the sperm will then be defrosted to room temperature in accordance with proper standard procedure.
● Surgical Sperm Extraction
The technique of surgical sperm extraction is targeted at azoospermia patients partaking in artificial insemination. Diverse surgical approaches for sperm extraction from the epididymis or testicle for in vitro fertilization will be implemented under a doctor’s assessment.
● Intrauterine Insemination, IUI
Intrauterine insemination, IUI is a method of conception that is closest to natural conception. It purifies high-quality and vital sperm by means of reproductive technology and is implanted into the uterine cavity with artificial assistance during the female ovulation period. In general, the success rate of a single intrauterine insemination is about 10 to 25%.
● Sperm DNA and mitochondrial examination
We apply bioinstrumentation to diagnose the cause of male infertility. The test items include oligozoospermia or azoospermia examinations, genetic micro-deletion of Y chromosome checkup, fragmentation detection of sperm DNA, and sperm mitochondrial membrane potential detection.
Fragmentation Detection of Sperm DNA
We devote ourselves in researching, developing, and modifying various types of methods for inspecting inherited abnormal genes, studying the DNA mechanism of reproductive medicine, and practicing the creation and therapeutic research of embryonic stem cells based on accurate direction and field of maternal and child health. In the meantime, we discuss the related issues with clinical doctors and reproduction medical teams periodically to strengthen the R&D capability and sustainability of our laboratory.
Embryonic Stem Cell Culture (ES cells)
In 2016, our animal center passed the on-site inspection and evaluation of an animal science application-based institution conducted by the Taichung City Animal Protection and Health Inspection Office, commissioned by the Council of Agriculture, Executive Yuan. Our facility features a reverse osmosis animal water supply system and high-efficiency IVC (individually ventilated cage) system with air filter system, forming a complete and safe breeding environment that provides diverse fodder made of different ingredients according to the physical requirements of different animals. The purpose is to maintain health management of our experiment animals while reducing the possibility of cross-contamination. In addition, we expect to ensure the accuracy of quality control and measurement of mouse embryo experiments in embryo culture fluid while providing studies related to reproductive medicine based on a mouse research model for boosting the rate of pregnancy.
Individually Ventilated Cage System(IVC)
Mouse Research Model