Knowledge Sharing

2010.09.07

Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility

2010 Annual Congress and international Symposium of Taiwan Association of Obstetrics and Gynecology. Abstract
Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility

Tsung-Hsien Lee 1,2 CAO,HUI-MEI 1, HONG MING-ZHOU 1, HUANG,JUN-JIA1, Zhong- Yi Chen 1, Maw-Sheng Lee1,2
Division of Infertility Clinic, Lee Women’s Hospital 1, Department of Obstetrics and Gynecology, Chung Shan Medical University 2

Specific Aim: To identify spermatozoa with apoptotic markers in couples with unexpected infertility (UI) and unsuccessful intrauterine insemination (IUI) and the efficiency and benefit of magnetic-activated cell sorting (MACS) in the elimination of apoptotic sperms and concentration of sperms with improved induced acrosome response.

Methods: Sixty couples of UI and two IUI failures were recruited. The sperms were prepared by conventional density gradient centrifugation (DGC) and equally divided into treatment and control groups. The treatment group was processed by MACS. Apoptotic rate, mitochondrial membrane potential (MMP) was analyzed by flow cytometry, and the fertilization potential was analyzed by induced acrosome reaction test (IART).

Results: Spermatozoa in the control group showed 13.5% apoptotic rate and 28.3% disrupted MMP. MACS decreased the apoptotic rate and disrupted MMP. Interestingly, the result of IART was significantly improvedafter magnetic sorting.

CONCLUSION: MACS may be helpful in improving the quality of spermatozoa for patients with UI and two IUI failures.