Knowledge Sharing

2010.09.07

Correlation between free radical content in follicular fluid and culture medium and embryo quality

2010 Annual Meeting of Taiwanese Society for Reproductive Medicine, Oral Report
Correlation between free radical content in follicular fluid and culture medium and embryo quality

Tsung-Hsien Lee *1,2,3 CAO,HUI-MEI 1, HUANG,JUN-JIA 1 Zhong- Yi Chen 1 Maw-Sheng Lee 1,2,3
Division of Infertility Clinic, Lee Women’s Hospital 1, Institute of Medicine, Chung Shan Medical University 2, Department of Obstetrics and Gynecology, Chung Shan Medical University 3

Specific Aim: To evaluate the association between free radical levels (ROS)in follicular fluid and culture medium and early-stage human embryo development.

Method: A total of 174 couples receiving assisted reproductive therapy between 2007 and 2008 were recruited in this study (IRB no.LWH08002). The ROS content in follicular fluid and culture medium was measured by luminometer. The percentage of good embryonic morphology and early-stage division rate were statistically analyzed.

Results: The oocyte with high ROS in the follicular fluid displayed poor quality at day 3 development after fertilization, especially during the ISCI treatment cycle. Intriguingly, high ROS content was also observed in embryos with poor development at day 3.

Conclusion: Measuring ROS content in follicular fluid and culture medium could be a useful parameter for IVF treatment.