Knowledge Sharing

2011.04.06

The comparison of fertilization rate and embryo quality of donor cryopreserved sperm with ICSI and convention IVF

2011 Taiwan Association of Obstetrics and Gynecology. Abstract
The comparison of fertilization rate and embryo quality of donor cryopreserved sperm with ICSI and convention IVF

CHEN XIU HUI*1, HUANG JUN-JIA 1,2, Zhong- Yi Chen 1, Tsung-Hsien Lee 4, HUANG LI-XIANG 1,3,Maw-Sheng Lee 1,4
1 Division of Infertility Clinic, Lee Women’s Hospital, 2 Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, 3 Institute of Medicine, Chung Shan Medical University, 4 Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital

Patients with azoospermia need donor sperm as a means to conceiving. However, to proven to be HIV-negative, donated semen need to be frozen and quarantined for 6 months before release for use. Therefore, intracytoplasmic sperm injection (ICSI) is a common clinical practice to ensure fertilization. According to the study in our hospital from 2004 to 2010, we found that when using qualified frozen sperm, higher fertilization rate was obtained by ICSI (80.8 ± 1.7%, p <0.01) as compared by conventional IVF (66.4 ± 2.4%). However, the yield of good quality embryos was higher in IVF (64.1 ± 3.4%, p = 0.022) as compared to ICSI (55.9 ± 3.0%). Therefore, conventional IVF is feasible for better embryo quality when using frozen sperm clinically, however, for the patients with low egg number, ICSI could be an alternative to ensure enough fertilization rate for embryo transfer.