Knowledge Sharing

2011.04.06

Nitric oxide affect early embryo development through S-nitrosylation of cytoplasmic protein

The 50th Annual Congress and international Symposium of Taiwan Association of Obstetrics and Gynecology. (2011 Annual Meeting)
Nitric oxide affect early embryo development through S-nitrosylation of cytoplasmic protein

Tsung-Hsien Lee 123, LIU CHONG XIAN 3, CAO,HUI-MEI 1, HUANG JUN-JIA 1, Zhong- Yi Chen 1, Maw-Sheng Lee 123
1 Division of Infertility Clinic, Lee Women’s Hospital, 2 Institute of Medicine, Chung Shan Medical University, 3 Department of Obstetrics and Gynecology, China Medical University Hospital

Specific Aim:To investigate the mechanisms of nitric oxide (NO) in the development and apoptosis of early-stage embryos.

Methods:Observation of early-stage mouse embryo development in in-vitro culture treated with NO donor or NOS inhibitor.

Results:The supplementation of NO donor disrupted embryo development and induced reduction of mitochondrial membrane potential, as well as elevated level of S-nitrosylation of cytoplasmic proteins. The addition of NOS inhibitor arrested embryo development but did not significantly affect the mitochondrial membrane potential, cytoplasmic protein S-nitrosylastion was lower in NOS inhibitor-treated embryos. Supplementation of anti-oxidants into culture medium was able to restore the quality of embryos.

CONCLUSION:The effect of NO donor on embryo development is closely related to the decline of mitochondrial membrane potential, as well as excess cytoplasmic protein S-nitrosylation and induced apoptosis. Supplementation of anti-oxidants into culture medium can help the embryo escape from the deleterious effect of NO.