Lee Women's Hospital presented the use of
spindle view technique combined with ICSI in a IVF treatment cycle for patients who had a fertilization rate of less than 50% in their previous ICSI treatment cycle. This significantly improved the fertilization rate. Statistical analysis revealed that in patients over 38 years old, the proportion of eggs with spindle deviation from the first polar body was higher. Therefore, the use of egg spindle localization technique combined with ICSI (intracytoplasmic sperm injection) can greatly enhance the fertilization rate in older women.
| Research Objective |
To analyze the spindle deviation angles of 335 women over 38 years old and their subsequent outcomes after ICSI. |
| Research Method |
The study utilized spindle view technique to analyze the spindle deviation angles of 1,222 eggs and grouped them accordingly. The patients were further divided into two groups based on their ovarian stimulation response, and the fertilization rate and the rate of good-quality D3 embryos were analyzed. A comparison was made between 22 patients who underwent egg spindle localization technique and 48 patients who underwent conventional intracytoplasmic sperm injection (ICSI). |
| Research Results |
For the group with spindle deviation angles ≤90° (79.1%-85.4%), the normal fertilization rate was significantly higher compared to the undetected group (59.5%) and the metaphase II group (61.5%). However, there was no significant difference observed among the groups in terms of the rate of good-quality Day 3 embryos (44.2%-70%). In the groups categorized as POR (poor ovarian response) and NOR (normal ovarian response), the fertilization rate of the undetected group (56.8%-65.8%) was lower than that of the group with spindle deviation angles ≤90° (79.0%-85.4%). |
Research Objective
To analyze the correlation between the Meiotic Spindle Deviation Angles (MSDA) of 335 women over 38 years old who received treatment at Lee Women's Hospital from January 2016 to May 2017 and the outcomes of intracytoplasmic sperm injection (ICSI).
Research Method
The study utilized egg spindle localization technique to analyze the spindle deviation angles of 1,222 eggs, divided into six groups: ≤15°, 16°-44°, 45°-90°, >90°, non-detection (ND), and telophase I (T1). Additionally, based on ovarian stimulation response, the patients were divided into two groups: normal ovarian responders (NOR) and poor ovarian responders (POR). A comparison was made between 22 patients who underwent egg spindle localization technique and 48 patients who underwent conventional intracytoplasmic sperm injection (ICSI).
Research Results
Out of the 1,222 eggs, spindle deviation signals were detected in 86.3% of the eggs.
Meiotic Spindle Deviation Angles and Day 3 Good Embryo Rate
For the group with spindle deviation angles ≤90° (79.1%-85.4%), the normal fertilization rate was significantly higher compared to the non-detection group (59.5%) and the telophase I group (61.5%). However, there was no significant difference in the Day 3 good embryo rate among the different spindle deviation angle groups (44.2%-70%).
| Spindle Deviation Angles |
Fertilized Embryos |
D3 Good-quality Embryos |
| ≤15° |
287 |
162（56.4%） |
| 16°-44° |
196 |
134（68.4%） |
| 45°-90° |
211 |
135（64.0%） |
| >90° |
26 |
15（57.7%） |
| Non-detection |
86 |
38（44.2%） |
| Telophase |
20 |
14（70.0%） |
| |
826 |
498（60.3%） |
Ovarian Response and Day 3 Good Embryo Rate
In the Poor Ovarian Responders (POR) and Normal Ovarian Responders (NOR) groups, the fertilization rate of the non-detection group (56.8%-65.8%) was lower than the group with spindle deviation angles ≤90° (79.0%-85.4%).
- NOR Group: There was no significant correlation between spindle deviation angles and the Day 3 good embryo rate.
- POR Group: The fertilization rate of the non-detection group (65.8%) was significantly lower than the group with spindle deviation angles ≤90° (70.0%-85.4%).
Additionally, in the group with spindle deviation angles of 16°-44°, both the fertilization rate (85.4%) and the Day 3 good embryo rate (69.4%) were significantly higher. Although the fertilization rates and clinical outcomes were similar whether or not the Spindle View technique was used, the POR group had a higher Day 3 good embryo rate when the technique was employed (63.0% vs. 40.9%).
| Spindle Deviation Angles |
Fertilized Embryos |
D3 Good-quality Embryos |
| ≤15° |
159（80.3%） |
73（54.1%） |
| 16°-44° |
111（85.4%） |
59（69.4%） |
| 45°-90° |
112（84.2%） |
44（53.0%） |
| >90° |
14（70.0%） |
6（50.0%） |
| Non-detection |
50（65.8%） |
17（39.5%） |
| Telophase |
7（46.7%） |
3（60.0%） |
| |
453（79.2%） |
202（55.6%） |
Reference: (2017, Aug). The effects of meiotic spindle deviation angles on fertilization and embryo quality in women over 38 years of age. Taiwan Society of Reproductive Medicine 2017 Annual Conference.
