How Does Egg Spindle Localization Technology Precisely Locate the Spindle?

Spindle View involves the precise positioning of the spindle in an egg before fertilization in IVF procedure to avoid damage during sperm injection. How does Spindle View achieve precise localization? What equipment is needed?
 

Mechanism and Characteristics of Spindle View

Spindle View involves the use of polarized light microscopy to observe the spindle inside the egg by utilizing its unique optical properties. Here is how polarized light microscopy of Spindle View helps accurately locate the egg spindle:
 

Characteristics of Spindle View
Properties of Polarization	Polarization refers to the light waves that vibrate in a single plane after passing through a filter. When polarized light passes through certain materials, it undergoes changes in intensity, direction, or phase. This property can be used to observe structures within the egg that exhibit consistent and organized arrangements.
Birefringence of Egg Spindle	The spindle inside the egg contains microtubules and exhibits birefringence. Birefringence refers to the property of a material that splits the incident light into two perpendicular components with different refractive indices. When polarized light interacts with the microtubules within the spindle, it causes a change in the polarization state of the light.
Observation with Polarized Filter	Polarized light microscopy contains polarizer and analyser. The polarized filter is placed below the sample, while the analyser filter is placed above the sample. These filters allow only specific directions of light to pass through and reach the observer’s eye.
Observation of the Spindle	By adjusting the relative rotation of the polarized filter and the analyser filter, the intensity and appearance of the spindle can be modified. The birefringence of the spindle causes it to appear as a bright or dark structure against the background. The direction and position of the spindle can be observed and determined based on its unique appearance.
Precise Spindle Localization	By manipulating the polarized filter and the analyser filter, embryologists can align the spindle in a way that maximizes its visibility and contrast. This allows for the accurate determination of the spindle’s location, orientation, and proximity to other structures within the egg.

Polarized light microscopy provides a non-invasive and real-time method for locating the spindle inside the egg. It can assist embryologists in precise localization during IVF procedure such as intracytoplasmic sperm injection (ICSI), thereby increasing the chances of successful fertilization and subsequent embryo development.

Polarized light microscopy required specialized equipment and expertise to accurately interpret the observed spindle structure. Experienced embryologists who have undergone training can effectively utilize this technology to achieve optimal results in IVF procedures.
 

Equipment of Spindle View

Spindle View requires specialized equipment to accurately locate the spindle and manipulate the egg. Here are some key equipment involved in Spindle View:
 

Microscope	High-quality microscopes are crucial for observing the spindle. These microscopes should have appropriate magnification capacity and optical devices to enable clear visualization of the spindle structures.
Polarized light microscopy	Polarized light microscopy involves the use of polarizer and analyzer to control the amplitude of light passing through the egg. Polarized filter is placed in the light path of the microscope, allowing for the precise position and orientation of the spindles by utilizing the principle of birefringence.
Specialized Glass Bottom Culture Dish	To observe the spindle based on birefringence, it is necessary to have a high transparency achieved through ultra-thin glass. Conventional plastic culture dishes are not suitable for spindle observation even with polarized light microscopy.
Microsurgical Manipulation Devices	Microsurgical Manipulation refers to precise manipulation of biological samples at a microscopic level. In Spindle View, microsurgical manipulation devices are used to stabilize the eggs and accurately position the ICSI pipette or other instruments used for ICSI.
Micromanipulation ICSI Pipette and Holding Pipette	ICSI pipette and holding pipette are used to stabilize the egg during the Spindle View localization process. They are extremely precise glass pipettes with controllable suction capabilities, allowing embryologists to hold and stabilize the eggs during ICSI procedure.

In addition to the hardware, environment conditions and the expertise of the operator also affect the success rate of Spindle View:

• Temperature and Environmental Control: Specialized environment control systems are used to ensure optimal conditions for Spindle View. These systems maintain stable temperature, humidity, and pH levels in the laboratory.
• Expertise and Training: The most critical aspect of Spindle View is the expertise and training of embryologists performing the procedure. Proper training and experience are crucial for accurately observing and manipulating the spindles, ensuring precise position and optimal fertilization outcomes.

Spindle View in IVF procedures requires the integration of advanced microscopy technique, microsurgical manipulation devices, and skilled embryologists trained in specific procedures. These tools and techniques allow for precise spindle localization and increase fertilization rate in IVF.